- 移动端
妙顺（上海）生物科技有限公司
16 年
手机商铺
商家活跃：
产品热度：
- NaN
- 0.5
- 1.5
- 0.5
- 3.5
推荐产品
公司新闻/正文
Roche的Dispase II小包装（1 g）现货供应
739 人阅读发布时间：2011-08-19 15:33
Roche的Dispase II小包装（1 g）现货供应
Dispase® II (neutral protease, grade II) nonsterile lyophilizate from Bacillus polymyxa
货号：4942078001，规格：1 g，价格：请询价。
供货商：Roche。货期：现货供应。
产品说明：
Benefits：
Enzyme activity: ≥0.8 U/mg (37°C, casein as substrate, pH 7.5)
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Specificity: Dispase is a nonspecific metalloprotease.
Working concentration: 0.6 to 2.4 U/ml
Preparation of Stock and Working Solution：
Dissolve the non-sterile lyophilized enzyme in Hepes-buffered saline (50 mM Hepes/KOH pH 7.4, 150 mM NaCl) (10 mg/ml). Dilute further with the culture medium to be used for the isolated cells to a final concentration of 0.6 to 2.4 U/ml. Concentrations higher than 2.4 U/ml are not recommended. Sterilize through a 0.22 μm filter membrane.
Dispase® II (neutral protease, grade II) nonsterile lyophilizate from Bacillus polymyxa
货号：4942078001，规格：1 g，价格：请询价。
供货商：Roche。货期：现货供应。
产品说明：
Application：
Dispase is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid and effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.Benefits：
- Rapid, effective, yet gentle agent that liberates cells with minimal cell damage
- Maintains cell membrane integrity
- Non-mammalian source - free of mycoplasma and animal virus contamination
- Extremely stable to influences of temperature, pH, and interference by serum components
- Easily inactivated by chelating agents or by dilution
- Delivers higher activity and convenience
Product Description：
EC 3.4.24.4Enzyme activity: ≥0.8 U/mg (37°C, casein as substrate, pH 7.5)
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Specificity: Dispase is a nonspecific metalloprotease.
Working concentration: 0.6 to 2.4 U/ml
Background Information：
One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 minute under assay conditions. A practical comparison of Roche units of Dispase® II with those cited in the Japanese literature (where dispase concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.Preparation of Stock and Working Solution：
Dissolve the non-sterile lyophilized enzyme in Hepes-buffered saline (50 mM Hepes/KOH pH 7.4, 150 mM NaCl) (10 mg/ml). Dilute further with the culture medium to be used for the isolated cells to a final concentration of 0.6 to 2.4 U/ml. Concentrations higher than 2.4 U/ml are not recommended. Sterilize through a 0.22 μm filter membrane.